Course Details

Return to Classes
Course Information
SemesterCourse Unit CodeCourse Unit TitleT+P+LCreditNumber of ECTS CreditsLast Updated Date
3BENG548CELL CULTURE TECHNIQUES3+0+037,514.05.2025

 
Course Details
Language of Instruction English
Level of Course Unit Master's Degree
Department / Program BIOENGINEERING
Type of Program Formal Education
Type of Course Unit Elective
Course Delivery Method Face To Face
Objectives of the Course - To introduce laboratory equipment, cell biology terminology and general information about the cell culture.
- To provide the knowledge of cell types together with the key principles for the maintenance of cells in vitro.
- To explain qualitative and quantitative characterization techniques in the cell culture.
- To describe 2D and 3D cell culture techniques and the use of biomaterials for the design of novel 3D cell cultures.
- To make students familiar with the transfection and hybridoma technologies.
- Providing the necessary background for understanding the fundamentals and applications of cell culture.
Course Content This course provides knowledge of basic cell culture concepts and terminology, and it aims to be a guide for the grad students to design and carry on both 2D and 3D in vitro experiments. The topics covered in this course include aseptic working area, maintenance of cells in the culture, cell-based assays, 2D/3D cell culture strategies, transfection and hybridoma technologies, and preventative and/or corrective actions for the contamination problem in the cell culture laboratory.
Course Methods and Techniques Active learning will be used in the course.
Prerequisites and co-requisities None
Course Coordinator None
Name of Lecturers Asist Prof.Dr. ZELİHA SORAN ERDEM zeliha.soranerdem@agu.edu.tr
Assistants None
Work Placement(s) No

Recommended or Required Reading
Resources Course slides
John Davis, “Animal Cell Culture: Essential Methods, ISBN: 978-0-470-97563-3, Wiley-Blackwell, 2011.
https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1563- PJT1267-COL31122-Gibco-Cell-Culture-Basics-Handbook-Global-FLR.pdf
https://www.vanderbilt.edu/viibre/CellCultureBasicsEU.pdf
Course Notes Course textbook, course slides.

Course Category
Science %30
Health %70

Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria
In-Term Studies Quantity Percentage
Quiz/Küçük Sınav 2 % 20
Ödev 2 % 15
Sunum/Seminer 1 % 25
Final examination 1 % 35
Diğer (Staj vb.) 1 % 5
Total
7
% 100

 
ECTS Allocated Based on Student Workload
Activities Quantity Duration Total Work Load
Grup Sunumu 1 0 0
Ev Ödevi 14 4 56
Sınıf İçi Aktivitesi 6 3 18
Sunum için Hazırlık 2 7 14
Sunum 2 5 10
Kısa Sınav 4 1 4
Araştırma 14 1 14
Ders dışı çalışma 14 1 14
Yüz Yüze Ders 14 3 42
Derse Devam 14 2 28
Final Sınavı 1 25 25
Total Work Load   Number of ECTS Credits 7,5 225

 
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
NoLearning Outcomes
1 Describe basic principles of cell culture and required equipment to establish a cell culture laboratory.
2 Define the key parameters to maintenance cells in vitro (such as media preparation, cryopreservation, thawing etc.) and the ability to optimize cell culture conditions for different cell types.
3 Explain how to troubleshoot in the cell culture laboratory.
4 Introduce the varying cell-based assays for the evaluation of cell health and quality.
5 Having knowledge of the difference between 2D and 3D cell cultures along with the strategies for 3D cell culture.
6 Discuss how to design an experiment in vitro for the academic research.

 
Weekly Detailed Course Contents
WeekTopicsStudy MaterialsMaterials
1 History and Introduction to Cell Culture
2 Working in a Cell Culture Laboratory: Aseptic Techniques and Safety Considerations
3 Types of Cells and Their Characteristics in the Culture
4 Stem Cell Types and Their Use in Cell Culture
5 Cell Culture Media and Growth Requirements for Animal and Stem Cells
6 Cryopreservation, Thawing, and Maintenance of the Cell Culture
7 Identification and Eradication of Common Contaminants in Cell Culture
8 Qualitative and Quantitative Assays in the Cell Culture
9 Transfection Techniques
10 Hybridoma Technology
11 3D Cell Culture Strategies and Biomaterials
14 Designing a Cell Culture Experiment

 
Contribution of Learning Outcomes to Programme Outcomes
P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12
All 4 3 3 4 4 3 2 4 2 2 3 4
C1 5 5 1 2 2 1 1 3 4 2 4 2
C2 4 2 4 4 2 3 1 2 1 1 3 4
C3 2 2 4 3 4 3 1 2 1 1 3 1
C4 2 2 2 5 4 1 2 2 2 1 2 1
C5 5 3 2 5 5 2 2 3 3 2 2 4
C6 5 4 4 5 4 5 4 4 3 4 1 4

  Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant

  
  https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77952&lang=en
Return to Classes