Course Details

Course Information

Semester	Course Unit Code	Course Unit Title	T+P+L	Credit	Number of ECTS Credits	Last Updated Date
2	BENG547	BIOCONJUGATE TECHNIQUES	3+0+0	3	7,5	13.05.2025

Course Details

Language of Instruction	English
Level of Course Unit	Master's Degree
Department / Program	BIOENGINEERING
Type of Program	Formal Education
Type of Course Unit	Elective
Course Delivery Method	Face To Face
Objectives of the Course	Identifying the chemistry of bioconjugates techniques. Describing the functional groups, determination of biological targets and potential modifications, and chemical reactions. Explaining the chemicals used in bioconjugation (functional cross linkers, tags, and probes) and their reaction mechanisms. Teaching the specific bioconjugate applications and their reactions.
Course Content	This course covers the determination of the functional groups/functions in biological systems and the chemical modifications and the reactions of these targets. In this course students learn Chemicals which are used in bioconjugation process (Functional chemical cross linkers, tags and probes) and their reactions. Bioconjugate applications such as bioconjugations and modifications are also covered.
Course Methods and Techniques	Face to face lecture, Discussion, Article presentation
Prerequisites and co-requisities	None
Course Coordinator	Asist Prof.Dr. İSMAİL AKÇOK ismail.akcok@agu.edu.tr
Name of Lecturers	Asist Prof.Dr. İSMAİL AKÇOK
Assistants	None
Work Placement(s)	No

Recommended or Required Reading

Resources

Functional targets Chemistry of reactive groups Zero-length Cross-linkers Homobifunctional, Heterobifunctional and Trifunctional Cross-linkers Cleavable Reagent Systems Tags and Probes Preparation of Hapten-Carrier Immunogen Conjugates Antibody Modification and Conjugation Immunotoxin Conjugation Techniques Preparation of Liposome Conjugates and Derivatives Avidin-Biotin Systems Modification with Synthetic Polymers, Enzyme Modification and Conjugation Nucleic acid and Oligonucleotide Modification and Conjugation

Course Notes

Bioconjugate Techniques, by Greg T. Hermanson.
Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Course Category

Engineering Design	%20
Science	%60
Health	%20

Planned Learning Activities and Teaching Methods

Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria

In-Term Studies	Quantity	Percentage
Quiz/Küçük Sınav	1	% 45
Sunum/Seminer	1	% 15
Final examination	1	% 40
Total	3	% 100

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration	Total Work Load
Araştırma Ödevi	5	5	25
Münazara	1	1	1
Tartışma	14	1	14
F2F Dersi	14	3	42
Sınıf İçi Aktivitesi	7	1	7
Sunum için Hazırlık	1	6	6
Sunum	5	1	5
Kısa Sınav	1	5	5
Okuma	14	2	28
Araştırma	14	3	42
Der Dışı Final Sınavı	1	10	10
Ders Dışı Ara Sınav	1	7	7
Total Work Load			Number of ECTS Credits 7,5 192

Course Learning Outcomes: Upon the successful completion of this course, students will be able to:

No	Learning Outcomes
1	Evaluate functional groups and biological targets with their reactions.
2	Analyze the chemical modifications of specific targets.
3	Identify the chemicals used in bioconjugation (cross-linkers, tags, and probes) and their reaction mechanisms.
4	Outline a current academic research
5	Develop a comprehensive knowledge for required experimental designs for academic research.

Weekly Detailed Course Contents

Week	Topics	Study Materials	Materials
1	Functional targets	Research, okuma	Bioconjugate Techniques, textbook
2	Chemistry of Reactive Groups	reading	Bioconjugate Techniques, textbook
3	Zero-length Cross-linkers	reading	Bioconjugate Techniques, textbook
4	Homobifunctional, Heterobifunctional and Trifunctional Cross-linkers	Reading, research	Bioconjugate Techniques, textbook
5	Cleavable Reagent Systems	Reading	Bioconjugate Techniques, textbook
6	Tags and Probes	Reading	Bioconjugate Techniques, textbook
7	Tags and Probes	Reading, Research	Bioconjugate Techniques, textbook
8	Preparation of Hapten-Carrier Immunogen Conjugates	Reading, Research	Bioconjugate Techniques, textbook
9	Antibody Modification and Conjugation	Reading, Research	Bioconjugate Techniques, textbook
10	Immunotoxin Conjugation Techniques	Reading, Research	Bioconjugate Techniques, textbook
11	Preparation of Liposome Conjugates and Derivatives / Avidin-Biotin Systems	Reading, Research	Bioconjugate Techniques, textbook
12	Modification with Synthetic Polymers	Reading, research	Bioconjugate Techniques, textbook
13	Enzyme Modification and Conjugation	Reading, Research	Bioconjugate Techniques, textbook
14	Nucleic acid and Oligonucleotide Modification and Conjugation	Reading	Bioconjugate Techniques, textbook
15
16

Contribution of Learning Outcomes to Programme Outcomes

	P1	P2	P3	P4	P5	P6	P7	P8	P9	P10	P11
All	3	1	2	3	1	2	1	1	1	1	1
C1	3	1	2	3	1	2
C2	3	1	2	3	1	2
C3	3	1	2	3	1	2
C4	3	1	2	3	1	2
C5	3	1	2	3	1	2

Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant

https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77951&lang=en

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