Course Details

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Course Information
SemesterCourse Unit CodeCourse Unit TitleT+P+LCreditNumber of ECTS CreditsLast Updated Date
1BENG545PROTEIN EXPRESSION&PURIFICATION3+0+037,514.05.2025

 
Course Details
Language of Instruction English
Level of Course Unit Master's Degree
Department / Program BIOENGINEERING
Type of Program Formal Education
Type of Course Unit Elective
Course Delivery Method Face To Face
Objectives of the Course O1. Defining pros and cons of protein expression systems
O2. Describing the basic principles of protein extraction and quantification methods and the outlining of chromatography methods
O3. Designing protein purification strategies
Course Content This course aims to deliver the overview of bacterial and eukaryotic protein expression systems and protein extraction methods from different sources. Principles of protein purification methods such as precipitation, centrifugation, electrophoresis and chromatography will be covered.
Course Methods and Techniques The course will be conducted using a student-centered teaching approach that emphasizes both theoretical understanding and applied knowledge. Fundamental concepts related to bacterial and eukaryotic protein expression systems, as well as protein extraction and purification methods, will be delivered through structured and interactive lectures. These sessions will incorporate visual materials and guided questioning to promote active participation. To deepen critical thinking and problem-solving skills, students will engage in problem-based learning activities, where they will analyze case studies and propose suitable strategies for protein purification under different scenarios. Group work will be integrated into the course, allowing students to collaboratively develop and present projects that simulate experimental workflows and methodological decision-making processes. Expert seminars by invited researchers or professionals from relevant fields will provide exposure to real-world applications and current advancements in protein science. In-class discussions and reflective exercises will be used to reinforce learning outcomes, promote the integration of knowledge, and foster analytical skills. Digital resources, including animations, articles, and virtual simulations, will support students’ independent study and enhance their comprehension of complex processes. Student presentations followed by structured peer and instructor feedback will further develop skills in scientific communication and critical evaluation.
Prerequisites and co-requisities None
Course Coordinator None
Name of Lecturers Asist Prof.Dr. EMEL BAŞAK GENCER AKÇOK emelbasak.gencerakcok@agu.edu.tr
Assistants None
Work Placement(s) No

Recommended or Required Reading
Resources 1. Protein Purification Techniques: A Practical Approach, Simon Roe, OUP Oxford 2001 2. Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition, Jan-Christer Janson, John Wiley & Sons, Inc., 2011
Course Notes 1. Protein Purification Techniques: A Practical Approach, Simon Roe, OUP Oxford 2001
2. Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition, Jan-Christer Janson, John Wiley & Sons, Inc., 2011
Documents Canvas
Assignments Canvas
Exams Sınıf içi

Course Category
Mathematics and Basic Sciences %100

Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria
In-Term Studies Quantity Percentage
Yarıyıl İçi Çalışmalarının Başarı Notunun Katkısı 1 % 30
Proje/Çizim 1 % 20
Sunum/Seminer 1 % 25
Final examination 1 % 25
Total
4
% 100

 
ECTS Allocated Based on Student Workload
Activities Quantity Duration Total Work Load
Grup Projesi 1 4 4
Ev Ödevi 4 3 12
Ders Dışı Sınav 1 5 5
Sunum 1 1 1
Proje 1 10 10
Kısa Sınav 1 6 6
Okuma 14 2 28
Araştırma 14 3 42
Kişisel Çalışma 14 2 28
Ders dışı çalışma 1 6 6
Yüz Yüze Ders 14 3 42
Final Sınavı 1 15 15
Total Work Load   Number of ECTS Credits 7,5 199

 
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
NoLearning Outcomes
1 design a protein purification strategy for a given protein.
2 criticize of a research paper reporting the protein expression and separation techniques.
3 learn chromatography instruments and columns.
4 participate in group discussions.

 
Weekly Detailed Course Contents
WeekTopicsStudy MaterialsMaterials
1 Introduction to recombinant DNA technology
2 Introduction to recombinant DNA technology
3 Protein and structure
4 PCR strategy, Cloning methods and Transformation
5 Expression systems; E.coli
6 Expression systems; Insect cells and Yeast
7 Expression systems; Mammalian cells
8 Extraction, clarification, sample preparation
9 Extraction, clarification, sample preparation
10 Protein purification and chromatography methods
11 Protein characterization methods
12 Protein characterization methods
13 Genetic Engineering
14 Genetic Engineering

 
Contribution of Learning Outcomes to Programme Outcomes
P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12
C1 5 5 5 4 4 3 4 4 4 4 5 5
C2 5 5 5 4 4 4 4 4 4 5 5 5
C3 5 5 5 5 5 5 4 4 4 4 5 5
C4 5 4 4 4 5 5 4 4 4 5 5 5

  Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant

  
  https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77949&lang=en
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