Course Details

Course Information

Semester	Course Unit Code	Course Unit Title	T+P+L	Credit	Number of ECTS Credits	Last Updated Date
1	BENG511	MOLECULAR BIOLOGY LABORATORY FOR ENGINEERS	3+0+0	3	7,5	11.02.2025

Course Details

Language of Instruction	English
Level of Course Unit	Master's Degree
Department / Program	BIOENGINEERING
Type of Program	Formal Education
Type of Course Unit	Elective
Course Delivery Method	Face To Face
Objectives of the Course	• To define the basics concepts and approaches for molecular bioengineering techniques • To apply the principles of molecular methods in understanding and regulating biological systems.
Course Content	The objective of this course is to introduce basic molecular biology techniques to graduate engineering students. Throughout the course, students will get insight into the scientific understanding and problem-solving abilities of an engineer. This course allows students to get technical knowledge and laboratory expertise for biological engineering research and design. The scope of the course includes laboratory safety and basic molecular biology techniques such as DNA isolation and sequencing, electrophoresis, transformation, polymerase chain reaction (PCR), ELISA and Western Blot etc.
Course Methods and Techniques	Quiz, Homework, Midterm and Final Exams
Prerequisites and co-requisities	None
Course Coordinator	Asist Prof.Dr. SİBEL SARI sibel.sari@agu.edu.tr
Name of Lecturers	Asist Prof.Dr. EMEL BAŞAK GENCER AKÇOK
Assistants	None
Work Placement(s)	No

Recommended or Required Reading

Resources

Lab safety Preparation of the solutions DNA isolation, quantification and characterization PCR\ sequencing Elisa Western Blot

Planned Learning Activities and Teaching Methods

Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria

Veri yok

ECTS Allocated Based on Student Workload

Veri yok

Course Learning Outcomes: Upon the successful completion of this course, students will be able to:

No	Learning Outcomes
1	Describe the fundamental aspects of molecular techniques and demonstrate the ability to carry out laboratory experiments
2	Examine the different methods and technologies for the analysis of DNA, RNA and protein
3	Interpret molecular data in peer-reviewed articles
4	Evaluate different molecular methodologies to interpret the results

Weekly Detailed Course Contents

Week	Topics	Study Materials	Materials
1	Brief Introduction to Molecular Biology
2	Introduction I: Lab safety
3	Introduction II: Preparation of the solutions
4	DNA isolation, quantification and characterization
5	Primer design
6	Transformation
7	PCR
8	Plasmid isolation from bacteria
9	Restriction enzymes
10	Ligation
11	Preparation for sequencing
12	Interpreting sequencing results
13	Elisa
14	Western Blot
15	Interpreting ELISA and WB results
16	Final Exam

Contribution of Learning Outcomes to Programme Outcomes

Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant

https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77927&lang=en