Course Details

RECOMBINANT DNA TECHNOLOGY

BENG618

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Course Information
SemesterCourse Unit CodeCourse Unit TitleT+P+LCreditNumber of ECTS Credits
1BENG618RECOMBINANT DNA TECHNOLOGY3+0+037,5

Course Details
Language of Instruction English
Level of Course Unit Master's Degree
Department / Program BIOENGINEERING
Type of Program Formal Education
Type of Course Unit Elective
Course Delivery Method Face To Face
Objectives of the Course Identifying theoretical knowledge of modern tools and techniques for manipulation and analysis of genomic sequences will be discussed.
Describing differences and pros or cons of techniques will be discussed to allow students to choose the most suitable one for a particular experimental set up.
Strategizing research methodologies employing genetic engineering techniques.
Emphasizing the application of recombinant DNA technology in biotechnological research.
Course Content DNA modifying enzymes, cloning strategies, vector types, selection and screening of recombinants, nucleic acid labeling techniques, genomic and cDNA library preparation, whole genome sequencing, the methods for site-directed mutagenesis and sequencing of cloned genomic fragments will be covered. Software permitting in-silico manipulation and annotation of DNA sequences for efficient design, tracking, and management of cloning experiments and the application of recombinant DNA technology in biotechnological research will be discussed.
Course Methods and Techniques
Prerequisites and co-requisities None
Course Coordinator Asist Prof.Dr. Ozkan Fidan ozkan.fidan@agu.edu.tr
Name of Lecturers Asist Prof.Dr. ÖZKAN FİDAN
Assistants None
Work Placement(s) No

Recommended or Required Reading
Resources

Course Category
Engineering %30
Science %70

Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria
In-Term Studies Quantity Percentage
Quiz/Küçük Sınav 1 % 30
Proje/Çizim 1 % 20
Sunum/Seminer 1 % 10
Final examination 1 % 40
Total
4
% 100

 
ECTS Allocated Based on Student Workload
Activities Quantity Duration Total Work Load
Tartışma 15 2 30
Ev Ödevi 5 2 10
Sunum 1 15 15
Proje 1 25 25
Okuma 15 3 45
Araştırma 15 2 30
Yüz Yüze Ders 15 3 45
Final Sınavı 1 25 25
Total Work Load   Number of ECTS Credits 7,5 225

Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
NoLearning Outcomes
1 Define versatile tools and techniques employed in genetic engineering and recombinant DNA technology.
2 Design and conduct experiments involving genetic manipulation.
3 Integrate how these are applied in basic and applied experimental biological research.
4 Discuss the topics from real life problems to solve.


Weekly Detailed Course Contents
WeekTopicsStudy MaterialsMaterials
1 Introduction
2 The manipulation of nucleic acids – basic tools and techniques
3 Isolation and separation of nucleic acids
4 Molecular analysis of nucleic acid sequences 1
5 Molecular analysis of nucleic acid sequences 2
6 Polymerase Chain Reaction
7 Midterm
8 Active Learning week
9 Nucleic acid sequencing methodologies
10 Introduction to cloning: Cloning vectors
11 Applications of gene cloning
12 Molecular Biotechnology and applications 1
13 Molecular Biotechnology and applications 2
14 Student Presentations
15 Final Exam


Contribution of Learning Outcomes to Programme Outcomes
P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12
All 5 3 2 4 4 5 2 4 3 3 4 4
C1 5 3 2 4 4 5 2 3 3 3 4 4
C2 5 3 2 4 4 5 3 3 3 3 4 4
C3 5 4 2 4 4 5 2 4 4 4 4 4
C4 5 4 3 4 5 5 2 5 3 3 4 4

Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant


https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=78038&lang=en
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