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Language of Instruction
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English
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Level of Course Unit
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Master's Degree
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Department / Program
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BIOENGINEERING
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Type of Program
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Formal Education
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Type of Course Unit
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Elective
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Course Delivery Method
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Face To Face
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Objectives of the Course
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Learning basic principles of mass spectrometry.
Evaluating pros and cons of variety of MS instruments.
Examining into applications of mass spectrometry in biological sciences.
Criticizing analysis mass spectrometry data.
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Course Content
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In this course basic concepts and principles of mass spectrometry, will be covered. Ion sources and ionization (ESI, APCI, FAB, MALDI and others), analyzers (Magnetic-Sector, Quadrupole, Time-of-Flight, Ion-trap, FT-ICR), and detectors. Interpretation of mass spectral data. Examples of mass spectrometry methodologies in different biological applications.
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Course Methods and Techniques
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This graduate-level course is designed to provide students with a comprehensive understanding of the basic concepts and principles of mass spectrometry. The course will cover various ion sources and ionization techniques, including Electrospray Ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI), Fast Atom Bombardment (FAB), Matrix-Assisted Laser Desorption/Ionization (MALDI), and others. Students will also explore the different types of analyzers such as Magnetic-Sector, Quadrupole, Time-of-Flight (TOF), Ion-trap, and Fourier Transform Ion Cyclotron Resonance (FT-ICR), alongside their corresponding detectors. The course will emphasize the interpretation of mass spectral data, allowing students to gain hands-on experience in analyzing complex biological samples. Real-world examples will be presented, highlighting the diverse applications of mass spectrometry methodologies in various biological fields, including proteomics, metabolomics, and biomarker discovery. Through this course, students will acquire a solid foundation in mass spectrometry techniques, enabling them to apply these methods in their own research projects.
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Prerequisites and co-requisities
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None
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Course Coordinator
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None
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Name of Lecturers
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Asist Prof.Dr. EMEL BAŞAK GENCER AKÇOK
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Assistants
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None
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Work Placement(s)
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No
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Recommended or Required Reading
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Resources
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Mass Spectrometry: A Textbook, Jürgen H. Gross
Quantitative Proteomics by Mass Spectrometry, Salvatore Sechi
Mass Spectrometry-Based Metabolomics: A Practical Guide Editörler: Sastia Prama Putri, Eiichiro Fukusaki
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canvas
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canvas
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sınıf içi
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