| Semester | Course Unit Code | Course Unit Title | T+P+L | Credit | Number of ECTS Credits | Last Updated Date |
| 1 | BENG618 | RECOMBINANT DNA TECHNOLOGY | 3+0+0 | 3 | 7,5 | 14.05.2025 |
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Language of Instruction
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English
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Level of Course Unit
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Doctorate's Degree
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Department / Program
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BIOENGINEERING
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Type of Program
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Formal Education
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Type of Course Unit
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Elective
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Course Delivery Method
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Face To Face
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Objectives of the Course
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Identifying theoretical knowledge of modern tools and techniques for manipulation and analysis of genomic sequences will be discussed.
Describing differences and pros or cons of techniques will be discussed to allow students to choose the most suitable one for a particular experimental set up.
Strategizing research methodologies employing genetic engineering techniques.
Emphasizing the application of recombinant DNA technology in biotechnological research.
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Course Content
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DNA modifying enzymes, cloning strategies, vector types, selection and screening of recombinants, nucleic acid labeling techniques, genomic and cDNA library preparation, whole genome sequencing, the methods for site-directed mutagenesis and sequencing of cloned genomic fragments will be covered. Software permitting in-silico manipulation and annotation of DNA sequences for efficient design, tracking, and management of cloning experiments and the application of recombinant DNA technology in biotechnological research will be discussed.
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Course Methods and Techniques
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Prerequisites and co-requisities
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None
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Course Coordinator
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Asist Prof.Dr. Ozkan Fidan ozkan.fidan@agu.edu.tr
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Name of Lecturers
|
Asist Prof.Dr. ÖZKAN FİDAN
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Assistants
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None
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Work Placement(s)
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No
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Recommended or Required Reading
Course Category
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Engineering
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%30
|
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Science
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%70
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Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
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In-Term Studies
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Quiz/Küçük Sınav
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1
|
%
30
|
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Proje/Çizim
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1
|
%
20
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Sunum/Seminer
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1
|
%
10
|
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Final examination
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1
|
%
40
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Total
|
4
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%
100
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ECTS Allocated Based on Student Workload
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Activities
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Total Work Load
|
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Tartışma
|
15
|
2
|
30
|
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Ev Ödevi
|
5
|
2
|
10
|
|
Sunum
|
1
|
15
|
15
|
|
Proje
|
1
|
25
|
25
|
|
Okuma
|
15
|
3
|
45
|
|
Araştırma
|
15
|
2
|
30
|
|
Yüz Yüze Ders
|
15
|
3
|
45
|
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Final Sınavı
|
1
|
25
|
25
|
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Total Work Load
| |
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Number of ECTS Credits 7,5
225
|
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
| No | Learning Outcomes |
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1
| Define versatile tools and techniques employed in genetic engineering and recombinant DNA technology. |
|
2
| Design and conduct experiments involving genetic manipulation. |
|
3
| Integrate how these are applied in basic and applied experimental biological research. |
|
4
| Discuss the topics from real life problems to solve. |
Weekly Detailed Course Contents
| Week | Topics | Study Materials | Materials |
| 1 |
Introduction
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| 2 |
The manipulation of nucleic acids – basic tools and techniques
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| 3 |
Isolation and separation of nucleic acids
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| 4 |
Molecular analysis of nucleic acid sequences 1
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| 5 |
Molecular analysis of nucleic acid sequences 2
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| 6 |
Polymerase Chain Reaction
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| 7 |
Midterm
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|
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| 8 |
Active Learning week
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| 9 |
Nucleic acid sequencing methodologies
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| 10 |
Introduction to cloning: Cloning vectors
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| 11 |
Applications of gene cloning
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| 12 |
Molecular Biotechnology and applications 1
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| 13 |
Molecular Biotechnology and applications 2
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|
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| 14 |
Student Presentations
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|
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| 15 |
Final Exam
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Recommended Optional Programme Components
Contribution of Learning Outcomes to Programme Outcomes
Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant
https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=78000&lang=en