| Semester | Course Unit Code | Course Unit Title | T+P+L | Credit | Number of ECTS Credits |
| 3 | BENG548 | CELL CULTURE TECHNIQUES | 3+0+0 | 3 | 7,5 |
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Language of Instruction
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English
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Level of Course Unit
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Master's Degree
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Department / Program
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BIOENGINEERING
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Type of Program
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Formal Education
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Type of Course Unit
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Elective
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Course Delivery Method
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Face To Face
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Objectives of the Course
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- To introduce laboratory equipment, cell biology terminology and general information about the cell culture.
- To provide the knowledge of cell types together with the key principles for the maintenance of cells in vitro.
- To explain qualitative and quantitative characterization techniques in the cell culture.
- To describe 2D and 3D cell culture techniques and the use of biomaterials for the design of novel 3D cell cultures.
- To make students familiar with the transfection and hybridoma technologies.
- Providing the necessary background for understanding the fundamentals and applications of cell culture.
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Course Content
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This course provides knowledge of basic cell culture concepts and terminology, and it aims to be a guide for the grad students to design and carry on both 2D and 3D in vitro experiments. The topics covered in this course include aseptic working area, maintenance of cells in the culture, cell-based assays, 2D/3D cell culture strategies, transfection and hybridoma technologies, and preventative and/or corrective actions for the contamination problem in the cell culture laboratory.
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Course Methods and Techniques
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Prerequisites and co-requisities
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None
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Course Coordinator
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Asist Prof.Dr. ZELİHA SORAN ERDEM zeliha.soranerdem@agu.edu.tr
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Name of Lecturers
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None
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Assistants
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None
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Work Placement(s)
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No
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Recommended or Required Reading
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Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
ECTS Allocated Based on Student Workload
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
| No | Learning Outcomes |
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1
| Describe basic principles of cell culture and required equipment to establish a cell culture laboratory. |
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2
| Define the key parameters to maintenance cells in vitro (such as media preparation, cryopreservation, thawing etc.) and the ability to optimize cell culture conditions for different cell types. |
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3
| Explain how to troubleshoot in the cell culture laboratory. |
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4
| Introduce the varying cell-based assays for the evaluation of cell health and quality. |
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5
| Having knowledge of the difference between 2D and 3D cell cultures along with the strategies for 3D cell culture. |
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6
| Discuss how to design an experiment in vitro for the academic research. |
Weekly Detailed Course Contents
Contribution of Learning Outcomes to Programme Outcomes
Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant
https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77952&lang=en