Course Details

BIOCONJUGATE TECHNIQUES

Course Information

Semester	Course Unit Code	Course Unit Title	T+P+L	Credit	Number of ECTS Credits
2	BENG547	BIOCONJUGATE TECHNIQUES	3+0+0	3	7,5

Course Details

Language of Instruction	English
Level of Course Unit	Master's Degree
Department / Program	BIOENGINEERING
Type of Program	Formal Education
Type of Course Unit	Elective
Course Delivery Method	Face To Face
Objectives of the Course	Identifying the chemistry of bioconjugates techniques. Describing the functional groups, determination of biological targets and potential modifications, and chemical reactions. Explaining the chemicals used in bioconjugation (functional cross linkers, tags, and probes) and their reaction mechanisms. Teaching the specific bioconjugate applications and their reactions.
Course Content	This course covers the determination of the functional groups/functions in biological systems and the chemical modifications and the reactions of these targets. In this course students learn Chemicals which are used in bioconjugation process (Functional chemical cross linkers, tags and probes) and their reactions. Bioconjugate applications such as bioconjugations and modifications are also covered.
Course Methods and Techniques
Prerequisites and co-requisities	None
Course Coordinator	Asist Prof.Dr. İSMAİL AKÇOK ismail.akcok@agu.edu.tr
Name of Lecturers	None
Assistants	None
Work Placement(s)	No

Recommended or Required Reading

Resources	Functional targets Chemistry of reactive groups Zero-length Cross-linkers Homobifunctional, Heterobifunctional and Trifunctional Cross-linkers Cleavable Reagent Systems Tags and Probes Preparation of Hapten-Carrier Immunogen Conjugates Antibody Modification and Conjugation Immunotoxin Conjugation Techniques Preparation of Liposome Conjugates and Derivatives Avidin-Biotin Systems Modification with Synthetic Polymers, Enzyme Modification and Conjugation Nucleic acid and Oligonucleotide Modification and Conjugation

Planned Learning Activities and Teaching Methods

Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria

Veri yok

ECTS Allocated Based on Student Workload

Activities	Quantity	Duration	Total Work Load
Araştırma Ödevi	1	4	4
Münazara	1	1	1
F2F Dersi	1	3	3
Sunum için Hazırlık	1	6	6
Sunum	1	1	1
Kısa Sınav	1	1	1
Okuma	1	1	1
Araştırma	1	4	4
Der Dışı Final Sınavı	1	10	10
Ders Dışı Ara Sınav	1	7	7
Total Work Load			Number of ECTS Credits 1,5 38

Course Learning Outcomes: Upon the successful completion of this course, students will be able to:

No	Learning Outcomes
1	Evaluate functional groups and biological targets with their reactions.
2	Analyze the chemical modifications of specific targets.
3	Identify the chemicals used in bioconjugation (cross-linkers, tags, and probes) and their reaction mechanisms.
4	Outline a current academic research
5	Develop a comprehensive knowledge for required experimental designs for academic research.

Weekly Detailed Course Contents

Week	Topics	Study Materials	Materials
1	Functional targets
2	Chemistry of Reactive Groups
3	Zero-length Cross-linkers
4	Homobifunctional, Heterobifunctional and Trifunctional Cross-linkers
5	Cleavable Reagent Systems
6	Tags and Probes
7	Tags and Probes
8	Preparation of Hapten-Carrier Immunogen Conjugates
9	Antibody Modification and Conjugation
10	Immunotoxin Conjugation Techniques
11	Preparation of Liposome Conjugates and Derivatives / Avidin-Biotin Systems
12	Modification with Synthetic Polymers
13	Enzyme Modification and Conjugation
14	Nucleic acid and Oligonucleotide Modification and Conjugation
15
16

Contribution of Learning Outcomes to Programme Outcomes

Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant

https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77951&lang=en