Semester | Course Unit Code | Course Unit Title | T+P+L | Credit | Number of ECTS Credits |
1 | BENG545 | PROTEIN EXPRESSION&PURIFICATION | 3+0+0 | 3 | 7,5 |
Language of Instruction
|
English
|
Level of Course Unit
|
Master's Degree
|
Department / Program
|
BIOENGINEERING
|
Type of Program
|
Formal Education
|
Type of Course Unit
|
Elective
|
Course Delivery Method
|
Face To Face
|
Objectives of the Course
|
O1. Defining pros and cons of protein expression systems
O2. Describing the basic principles of protein extraction and quantification methods and the outlining of chromatography methods
O3. Designing protein purification strategies
|
Course Content
|
This course aims to deliver the overview of bacterial and eukaryotic protein expression systems and protein extraction methods from different sources. Principles of protein purification methods such as precipitation, centrifugation, electrophoresis and chromatography will be covered.
|
Course Methods and Techniques
|
|
Prerequisites and co-requisities
|
None
|
Course Coordinator
|
None
|
Name of Lecturers
|
Asist Prof.Dr. EMEL BAŞAK GENCER AKÇOK emelbasak.gencerakcok@agu.edu.tr
|
Assistants
|
None
|
Work Placement(s)
|
No
|
Recommended or Required Reading
|
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
ECTS Allocated Based on Student Workload
Activities
|
Total Work Load
|
Ara Teslim ve Jüri
|
1
|
10
|
10
|
F2F Dersi
|
1
|
3
|
3
|
Grup Projesi
|
1
|
4
|
4
|
Ev Ödevi
|
1
|
2
|
2
|
Sunum
|
1
|
2
|
2
|
Proje
|
1
|
2
|
2
|
Kısa Sınav
|
1
|
3
|
3
|
Okuma
|
1
|
2
|
2
|
Araştırma
|
1
|
3
|
3
|
Kişisel Çalışma
|
1
|
2
|
2
|
Teslim
|
1
|
4
|
4
|
Ders dışı çalışma
|
1
|
6
|
6
|
Yüz Yüze Ders
|
1
|
3
|
3
|
Final Sınavı
|
1
|
15
|
15
|
Total Work Load
| |
|
Number of ECTS Credits 2
61
|
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
No | Learning Outcomes |
1
| design a protein purification strategy for a given protein. |
2
| criticize of a research paper reporting the protein expression and separation techniques. |
3
| learn chromatography instruments and columns. |
4
| participate in group discussions. |
Weekly Detailed Course Contents
Contribution of Learning Outcomes to Programme Outcomes
Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant
https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77949&lang=en