| Semester | Course Unit Code | Course Unit Title | T+P+L | Credit | Number of ECTS Credits |
| 1 | BENG511 | MOLECULAR BIOLOGY LABORATORY FOR ENGINEERS | 3+0+0 | 3 | 7,5 |
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Language of Instruction
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English
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Level of Course Unit
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Master's Degree
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Department / Program
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BIOENGINEERING
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Type of Program
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Formal Education
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Type of Course Unit
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Elective
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Course Delivery Method
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Face To Face
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Objectives of the Course
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• To define the basics concepts and approaches for molecular bioengineering techniques
• To apply the principles of molecular methods in understanding and regulating biological systems.
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Course Content
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The objective of this course is to introduce basic molecular biology techniques to graduate engineering students. Throughout the course, students will get insight into the scientific understanding and problem-solving abilities of an engineer. This course allows students to get technical knowledge and laboratory expertise for biological engineering research and design. The scope of the course includes laboratory safety and basic molecular biology techniques such as DNA isolation and sequencing, electrophoresis, transformation, polymerase chain reaction (PCR), ELISA and Western Blot etc.
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Course Methods and Techniques
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Quiz, Homework, Midterm and Final Exams
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Prerequisites and co-requisities
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None
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Course Coordinator
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Asist Prof.Dr. SİBEL SARI sibel.sari@agu.edu.tr
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Name of Lecturers
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None
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Assistants
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None
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Work Placement(s)
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No
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Recommended or Required Reading
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Resources
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Lab safety
Preparation of the solutions
DNA isolation, quantification and characterization
PCR\ sequencing
Elisa
Western Blot
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Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
ECTS Allocated Based on Student Workload
Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
| No | Learning Outcomes |
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1
| Describe the fundamental aspects of molecular techniques and demonstrate the ability to carry out laboratory experiments |
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2
| Examine the different methods and technologies for the analysis of DNA, RNA and protein |
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3
| Interpret molecular data in peer-reviewed articles |
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4
| Evaluate different molecular methodologies to interpret the results |
Weekly Detailed Course Contents
| Week | Topics | Study Materials | Materials |
| 1 |
Brief Introduction to Molecular Biology
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| 2 |
Introduction I: Lab safety
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Introduction II: Preparation of the solutions
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DNA isolation, quantification and characterization
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Primer design
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Transformation
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| 7 |
PCR
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| 8 |
Plasmid isolation from bacteria
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| 9 |
Restriction enzymes
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| 10 |
Ligation
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Preparation for sequencing
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Interpreting sequencing results
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Elisa
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Western Blot
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Interpreting ELISA and WB results
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| 16 |
Final Exam
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Contribution of Learning Outcomes to Programme Outcomes
Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant
https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=77927&lang=en