Course Details

RECOMBINANT DNA TECHNOLOGY

BENG310

Course Information
SemesterCourse Unit CodeCourse Unit TitleT+P+LCreditNumber of ECTS Credits
5BENG310RECOMBINANT DNA TECHNOLOGY3+0+035

Course Details
Language of Instruction English
Level of Course Unit Bachelor's Degree
Department / Program BIOENGINEERING
Type of Program Formal Education
Type of Course Unit Elective
Course Delivery Method Face To Face
Objectives of the Course The main purpose of this course is to provide the students with an insight into techniques relevant to cell and molecular biology
Course Content The course will cover gene primer designing, databases for finding gene and gene sequence, restriction enzymes, gene cloning, transfection/transformation, DNA delivery, Polymerase Chain Reactions, cell culture, and gene editing technologies (CRISPR, TALEN, etc.). Chip-seq, single-cell RNA-sequence. This knowledge enables students to
understand the basic principles of techniques used in molecular biology and genetics.
Course Methods and Techniques The course is based on a student-centered approach that incorporates in-class activities, group work, homework assignments, mini projects, and technical trips. Various interactive methods such as discussions, peer collaboration, and hands-on applications will be employed. The teaching methodology also includes individual presentations and integration of technology-enhanced learning tools to support critical thinking and practical understanding.
Prerequisites and co-requisities None
Course Coordinator None
Name of Lecturers Asist Prof.Dr. Mesude Biçer mesude.bicer@agu.edu.tr
Assistants None
Work Placement(s) No

Recommended or Required Reading
Resources Recombinant DNA Technology - Keya Chaudhuri
Lecture notes and reading materials will be shared weekly through the CANVAS platform. The main textbook is:

Andreas Maier, Stefan Steidl, Vincent Christlein, Joachim Hornegger, Medical Imaging Systems, Springer Open, 2018.

Additionally, scientific articles from journals and conference proceedings such as IEEE TIP, ICIP, and CVPR will be recommended throughout the course.
Lecture notes and reading materials will be shared weekly through the CANVAS platform. The main textbook is: Andreas Maier, Stefan Steidl, Vincent Christlein, Joachim Hornegger, Medical Imaging Systems, Springer Open, 2018.
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Course Category
Mathematics and Basic Sciences %10
Engineering %15
Engineering Design %10
Social Sciences %0
Education %5
Science %15
Health %30
Field %60

Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"

Assessment Methods and Criteria
In-Term Studies Quantity Percentage
Yarıyıl İçi Çalışmalarının Başarı Notunun Katkısı 1 % 5
Quiz/Küçük Sınav 1 % 10
Ödev 3 % 20
Proje/Çizim 1 % 30
Final examination 1 % 35
Total
7
% 100

 
ECTS Allocated Based on Student Workload
Activities Quantity Duration Total Work Load
Araştırma Ödevi 4 3 12
Tartışma 10 3 30
Sınıf İçi Aktivitesi 8 3 24
Sunum 1 1 1
Araştırma 3 2 6
Takım/Grup Çalışması 1 1 1
Yüz Yüze Ders 14 3 42
Final Sınavı 1 4 4
Total Work Load   Number of ECTS Credits 4 120

Course Learning Outcomes: Upon the successful completion of this course, students will be able to:
NoLearning Outcomes
1 Explain how to clone a gene of interest
2 Interpret about gene editing systems
3 Advance online tools related to molecular biology and genetics
4 Define Chip-seq, single cell RNA-sequence.


Weekly Detailed Course Contents
WeekTopicsStudy MaterialsMaterials
1 Finding gene and gene sequence
2 Restriction enzymes
3 Gene Cloning
4 Gene editing: CRIPSR
5 PCR
6 How to design the diagnostic PCR tests
7 RT-PCR
8 Chip-Seq
9 Chip-Seq
10 Bioinformatic tools for single cell RNA-sequence
11 Bioinformatic tools for single cell RNA-sequence
12 Bioinformatic tools for single cell RNA-sequence
13 Bioinformatic tools for single cell RNA-sequence
14 FINAL


Contribution of Learning Outcomes to Programme Outcomes
P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12
C1 5 5 5 5 5 3 5
C2 5 5 5 5 5 3 5 3 3 3 3 3
C3 5 5 5 5 5 5 5
C4 5 5 5 5 5 5 5

Contribution: 1: Very Slight 2:Slight 3:Moderate 4:Significant 5:Very Significant


https://sis.agu.edu.tr/oibs/bologna/progCourseDetails.aspx?curCourse=71982&lang=en