| Week | Topics | Study Materials | Materials |
| 1 |
Introduction to Bioengineering Lab 2
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| 2 |
PCR amplification of the gene,
Recovery of PCR product
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| 3 |
Gel purification,
Ligation of PCR production into cloning vector,
Transformation into E. coli XL1-Blue
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| 4 |
Colony pickup and plasmid extraction,
Checking correct plasmid using restriction digest enzymes,
Transformation of correct plasmid and expression plasmid into E. coli XL1-Blue
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| 5 |
Colony pickup and plasmid extraction,
Restriction digest of the plasmids and checking them on the gel,
Recovery of insert and vector from the gel
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| 6 |
Ligation of insert into vector,
Transformation of ligation mixture into E. coli Xl1-Blue
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| 7 |
Fall Break
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| 8 |
Colony pickup and plasmid extraction,
Checking correct plasmid using restriction digest enzymes,
Transformation of correct expression plasmid into E. coli BL21(DE3),
Media Preparation in flasks
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| 9 |
Colony pickup,
Inoculation of seed culture into flask,
Induction
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| 10 |
Extraction of cell broth with organic solvent,
Analysis of the extract in HPLC,
Protein expression check with SDS-PAGE
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| 11 |
Yeast DNA Isolation
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| 12 |
PCR amplification, and Gel Electrophoresis
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| 13 |
Preparation of media and bioreactors under batch fermentation to bioprocess P. kudriavzevii
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| 14 |
Fed batch fermentation to grow P. kudriavzevii biomass
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| 15 |
Downstream bioprocess development of P. kudriavzevii
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| 16 |
Final exam
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